The effect of the shape on the affinity is obvious in medium group.
The interface areas of CHL4-AME1 and IML3-CTF3 are similar; however, the hydrophobicity in IML3-CTF3 interface is significantly higher, also the binding area is slightly deep in groove shape while in CHL4-AME1 is quite flat, thus the affinity of IML3 and CTF3 is significantly higher than the affinity between CHL4 and AME1.
Subunits AME1, CTF19, MCM21 and OKP1 can form an important inner kinetochore assembly, which is conserved between budding yeasts and humans [1,2].
The cells without CTF3 exhibit prolonged prometaphase delay in mitosis, which leads to the failure of cytokinesis and fast cell death. This is due to the loss of inner kinetochore components, such as MIF2 and MCM16. Besides, it is also caused by the failure of the spindle checkpoint response [3].
The shape of CHL4-AME1 is quite flat !
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IML3-CTF3 interface is slightly deep in groove shape!
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[1] Alexander Schleiffer et al., 2012. CENP-T proteins are conserved centromere receptors of the Ndc80 complex. Nature Cell Biology, 14(6), pp.604–613.
[2] Schmitzberger, F. et al., 2017. Molecular basis for inner kinetochore configuration through RWD domain–peptide interactions. The EMBO Journal, 36(23), pp.3458–3482.
[3] Perpelescu, M. & Fukagawa, T., 2011. The ABCs of CENPs. Chromosoma, 120(5), pp.425–446.
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