A relatively well-conserved CHL4 C-terminal
The percentage of conserved residues at the CHL4 (Cenp-N) C-terminal domain are indicated in different colours. In this region of the CCAN complex, almost 60% of the residues are relatively well-conserved.
Certain specific functionality or crucial interactions are predicted to occur at highly conserved amino acid positions.
Relative Conservation Plot of Aligned Sequences
The majority of C-terminal residues fall within the areas of relatively low conservation. However, a higher conservation can be found at the amino acids positions where protein-protein interactions take place.
CHL4:MCM21 (N:O) and CHL4:CTF19 (N:P) interface involves amino acid at similar positions (or the same residues could interact with both MCM21 and CTF19) . Therefore, these two together results in a relative conservation as high as 0.07.