❓Why Should We Study The Conservation of CHL4 N-terminal?
The sequence in the proteome of the evolutionary lineage will gradually change due to random mutation or deletion [1], and the conserved sequence is a sequence that can remain relatively stable and persist in this case.
The analysis of conservation can help us understand the homology of the protein and the important functions of the conserved regions.
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※Intuitively, highly conserved and moderately conserved regions occupy most of the chain.
The sequences of centromere protein N from 13 different mammals, plants and microorganisms were selected for comparison to determine the conservation of CHL4 using analyzing tools Consurf and T-COFFEE.
As a result, we can see that CHL4 N-terminal is relatively conservative. The results of Consurf and T-COFFEE are somewhat different but within acceptable limits.
※Most Interface Residues Have Average to Good Conservation.
The residues involved in the formation of Van der Waals forces have no direct relationship with conservation.
Most of the residues involved in the formation of hydrogen bonds and salt bridges are highly conserved (as shown below), except for Ser229 and Ser164.
Most of the residues involved in the formation of hydrogen bonds are conserved, which can be deduced that there is a certain relationship between conservation and hydrophilicity.
*The data were generated by NCBI and Uni-prot and then analysed by Consurf and T-COFFEE websites.
[1] Kimura M. Evolutionary rate at the molecular level[J]. Nature, 1968, 217(5129): 624-626.
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