Binding affinity of interfaces

Before investigating the contribution of each interaction,  through a web service PRODIGY (PROtein binDIng enerGY prediction)¹, the binding affinity (indicated by ∆G˚' and K_d values) of each interfaces related to CTF3, MCM21 and CTF19 were predicted (as shown in tables in next page). Based on this mathematical model, more realistic ∆G˚' and K_d values can be predicted¹.

Predictive model used by PRODIGY

ICs: Numbers of Interfacial Contacts found at the interface;

NIS: Non-Interacting Surface. 

The predictive model includes the effect from non-interacting surface (NIS), which also has contribution to the binding affinity between proteins.¹

*More information of the predictive model can be found on this website: Methodology (👈Click it!).

A colour-coded list of protein names and their corresponding subunit names.

Reference

1.        Vangone A, Bonvin AMJJ. Contacts-based prediction of binding affinity in protein–protein complexes. Elife. 2015;4(JULY2015). doi:10.7554/eLife.07454