Small interface

Here we are going to focus on five small interfaces with interface area below 500A. The overview of interfaces from each complex is shown in the main page. Some interesting comparisons between complexes that demonstrate the influence of entropy upon affinity can be found in the links below.

 

MCM21: NKP2 // CTF19: NKP1          MCM21: NKP1 // MCM21: OKP1          MCM21: NKP1 // CTF3: CTF19

MCM21:NKP2 interface

 

 

Interface area: 205.1 A: 213.2 A

Hydrophobic interface area: 143.8 A: 81.01 A (70%:38%)

Biological importance: This complex is part of a larger subcomplex in CCAN, but it is not functional as a dimer. As MCM21 works closely with the tetramer, COMA; NKP2 dimerises with NKP1 to stabilise the COMA [1].

CFT19: NKP1 interface

 

 

Interface area: 242 A: 223 A

Hydrophobic interface area: 72.98 A: 41.61 A (30%: 19%)

Biological importance: CTF19 is part of the COMA complex, that does not form a functional dimer with NKP1. For details please see MCM21:NKP2 interface.

MCM21: NKP1 interface

 

 

Interface area: 281.2 A: 310.7 A

Hydrophobic interface area: 165.09 A: 50.20 A (59%: 16%)

Biological importance: The complex is not a functional dimer. For details please see MCM21:NKP2 interface

MCM21: OKP1 interface

 

 

Interface area: 290 A: 293.5 A

Hydrophobic interface area: 82.28 A: 45.81 A (28%: 16%)

Biological importance: The complex is part of the COMA comlex, but it does not form a functional dimer.  For details please see CTF19: OKP1 interface

CTF3: CTF19 interface

Interface area: 290 A: 306 A

Hydrophobic interface area: 161.89 A: 146.54A (56%: 48%)

Biological importance: Subunits involved in this complex belong to two different CCAN subcomplexes. However, it was shown that the recruitment of CTF3 to CCAN is dependent on the complex formed by CTF19: MCM21 [1]. 

1. Hinshaw S, Harrison S. The structure of the Ctf19c/CCAN from budding yeast. eLife. 2019;8.