Large interface

Five large interfaces are focused on here. The overview of the interface is shown in this page below. Detailed information on how their interface property contributes to affinity is shown in the following links.

 

CTF19: MCM21 // MCM16: CTF3             CTF3: MCM22 // CTF19: AME1

CTF19: OKP1 interface

 

Total interface area: 986.4A : 1037.9A

Hydrophobic interface area: 602.02A : 624.29A (61%: 60%)

Biological importance: The complex is part of the tetramer called COMA, which connects the inner kinetochore proteins with the outer kinetochore. However individually, they form a more closely interacting functional dimer with other proteins. CTF19 dimerise with MCM21 and OKP1 with AME1. The former complex plays a regulatory role in recruiting other inner kinetochore proteins, whereas the latter recruits outer kinetochore proteins and interacts with DNA [1].

CTF19: AME1 interface

 

Total interface area: 1241.8A : 1284.4A

Hydrophobic interface area: 581.12A : 629.3A (47%: 49%)

Biological importance: Not the functional dimer in the COMA complex, for details please see CTF19: OKP1 interface

CTF3: MCM22 interface

 

Total interface area: 1210A : 1226A

Hydrophobic interface area: 575.07A : 711.81A (48%: 58%)

Biological importance: CTF3, MCM22 and MCM16 forms a stable functional trimer, together with another dimer to recruit theNdc80 complex, which is responsible for connecting microtubules [1].

CTF19: MCM21 interface

 

Total interface area: 2203.5A : 2245.3A

Hydrophobic interface area: 1309.75A : 1644.64A (60%: 73%)

Biological importance: Functional dimer in the COMA complex, for details please see CTF19: OKP1 interface

MCM16: CTF3 interface

 

Total interface area: 2337A : 2256A

Hydrophobic interface area: 1078.85A : 1301.65A (46%: 58%)

Biological importance: part of the functional trimer, for details please see CTF3: MCM22 interface

1. Hinshaw S, Harrison S. The structure of the Ctf19c/CCAN from budding yeast. eLife. 2019;8.